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Faculté des Sciences appliquées
Faculté des Sciences appliquées
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Elaboration of a structured biomaterial to be used as a substrate for animal cell culture

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Tilkin, Rémi ULiège
Promotor(s) : Grandfils, Christian ULiège
Date of defense : 28-Jun-2016 • Permalink : http://hdl.handle.net/2268.2/1405
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Title : Elaboration of a structured biomaterial to be used as a substrate for animal cell culture
Translated title : [fr] Elaboration d'un biomatériau structuré destiné à servir de substrat pour la culture de cellules animales
Author : Tilkin, Rémi ULiège
Date of defense  : 28-Jun-2016
Advisor(s) : Grandfils, Christian ULiège
Committee's member(s) : Geris, Liesbet ULiège
Lambert, Stéphanie ULiège
Lambert, Charles ULiège
Keck-Antoine, Klaus ULiège
Toye, Dominique ULiège
Language : English
Number of pages : 96
Keywords : [en] cell culture
[en] cell adhesion
[en] cell proliferation
[en] biomaterials
[en] polystyrene
[en] polylactide
[en] hydrolysis
[en] aminolysis
[en] fibroblast
[en] osteoblast
Discipline(s) : Engineering, computing & technology > Chemical engineering
Funders : CEIB
Research unit : CEIB
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en ingénieur civil en chimie et science des matériaux, à finalité approfondie
Faculty: Master thesis of the Faculté des Sciences appliquées

Abstract

[en] The global scope of this work was the study of adhesion and proliferation of fibroblasts L-929 and osteoblasts MG-63 on untreated polystyrene, tissue culture polystyrene, untreated polylactide, hydrolyzed polylactide, and aminolyzed polylactide. A state of the art and techniques was first realized. Substrates were then produced. Untreated and treated polystyrene were cut into disks while polylactide powder was pressed into disks, after optimization of the production process,. The surface of disks was chemically modified by hydrolysis and aminolysis surface treatment. Substrates were characterized by optical microscopy, SEM, and water contact angle. Finally, cell adhesion, proliferation, and viability on these substrates were assessed by cell counting and MTT assay after 1, 4, and 8 days of cell culture. Results from surface characterization showed an increase of roughness for hydrolyzed polylactide. Regarding water contact angles measurements, values were smaller on treated substrates even though the difference with untreated substrates appeared smaller than expected. Cell counting and MTT assay showed an increase of cell proliferation and cell viability for treated polylactide substrates in the case of fibroblasts and a decrease of cell proliferation for aminolyzed polylactide substrates in the case of osteoblasts. After comparison with a commercial membrane called Alvetex, it was hypothesized that the Alvatex was a suitable substrate for fibroblast culture. Future developments were also proposed such as the optimization of temperature for press molding process during PLA plates production, a deeper characterization of surface chemistry and roughness, and the investigation of the effect of floating substrates on cell proliferation.


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Author

  • Tilkin, Rémi ULiège Université de Liège > Master ingé. civil chim. & sc. mat., fin. appr. (ex 2e ma.)

Promotor(s)

Committee's member(s)

  • Geris, Liesbet ULiège Université de Liège - ULg > Département d'aérospatiale et mécanique > Génie biomécanique
    ORBi View his publications on ORBi
  • Lambert, Stéphanie ULiège Université de Liège - ULg > Department of Chemical Engineering > Department of Chemical Engineering
    ORBi View his publications on ORBi
  • Lambert, Charles ULiège Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
    ORBi View his publications on ORBi
  • Keck-Antoine, Klaus ULiège Université de Liège - ULg > Department of Chemical Engineering > Procédés industriels de polymérisation
    ORBi View his publications on ORBi
  • Toye, Dominique ULiège Université de Liège - ULg > Department of Chemical Engineering > Génie de la réaction et des réacteurs chimiques
    ORBi View his publications on ORBi
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