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SEA Ligation Assisted Preparation of Cyclic Peptides Under Microfluidic Conditions

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Toupy, Thomas ULiège
Promotor(s) : Monbaliu, Jean-Christophe ULiège ; Melnyk, Oleg
Date of defense : 27-Jun-2017 • Permalink : http://hdl.handle.net/2268.2/2478
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Title : SEA Ligation Assisted Preparation of Cyclic Peptides Under Microfluidic Conditions
Translated title : [fr] Préparation de Peptides Cycliques Assistée par Ligation SEA Sous Conditions Microfluidiques
Author : Toupy, Thomas ULiège
Date of defense  : 27-Jun-2017
Advisor(s) : Monbaliu, Jean-Christophe ULiège
Melnyk, Oleg 
Committee's member(s) : Quinton, Loïc ULiège
Damblon, Christian ULiège
Language : English
Number of pages : 83
Keywords : [en] SEA Ligation
[en] Peptides
[en] Cyclic Peptides
[en] Microfluidic
Discipline(s) : Physical, chemical, mathematical & earth Sciences > Chemistry
Research unit : Center for Integrated Technology and Organic Synthesis (CiTOS)
Institut de Biologie de Lille (IBL)
Target public : Researchers
Professionals of domain
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en sciences chimiques, à finalité approfondie
Faculty: Master thesis of the Faculté des Sciences

Abstract

[en] The intramolecular SEA ligation for the preparation of cyclic peptides of various sizes was studied under microfluidic conditions. Two type of peptides, namely POL (10 residues) and RTD-1 (18 residues) as well as difficult junctions (e.g. valine, isoleucine, and threonine) were chosen as the main targets of this research. The microfluidic setup for the intramolecular SEA ligation formally telescoped two process steps. In the first microreactor, the reversible N,S-acyl shift reaction lead to the reactive SEA thioester species in ~90% conversion for all studied peptides. The telescoping of the first step and the use of the reactive SEA thioester species in the capture step of the N- terminal cysteine mediated by an exogenous arylthiol catalyst produced a cyclic thioester intermediate which upon irreversible S,N-acyl shift gave the final cyclic product. This fully telescoped process of 64 (POL and analogs) to 75 min (RTD-1 and analogs) total residence time gave the associated desired cyclic product with conversions ranging from 60 to 96% depending on the peptide sequence and the ligation site.


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  • Toupy, Thomas ULiège Université de Liège > Master en sc. chimiques, à fin.

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