Optimisation de matériaux hybrides polymères/céramiques destinés à l'ingénierie tissulaire
Colle, Xavier
Promotor(s) :
Grandfils, Christian
;
Lambert, Stéphanie
Date of defense : 25-Jun-2018/26-Jun-2018 • Permalink : http://hdl.handle.net/2268.2/4630
Details
Title : | Optimisation de matériaux hybrides polymères/céramiques destinés à l'ingénierie tissulaire |
Translated title : | [en] Optimization of polymeric / ceramic hybrid materials for tissue engineering |
Author : | Colle, Xavier ![]() |
Date of defense : | 25-Jun-2018/26-Jun-2018 |
Advisor(s) : | Grandfils, Christian ![]() Lambert, Stéphanie ![]() |
Committee's member(s) : | Ruffoni, Davide ![]() Habraken, Anne ![]() Tilkin, Rémi ![]() Léonard, Angélique ![]() |
Language : | French |
Number of pages : | 79 |
Discipline(s) : | Engineering, computing & technology > Materials science & engineering |
Institution(s) : | Université de Liège, Liège, Belgique |
Degree: | Master en ingénieur civil en chimie et science des matériaux, à finalité spécialisée |
Faculty: | Master thesis of the Faculté des Sciences appliquées |
Abstract
[en] The aim of this exploratory study was to assess the efficiency of incorporation and the release of a model protein, the soybean trypsin inhibitor (STI), in silica gels synthesized by the sol gel method. The protein has been incorporated into the gels by two distinct methods:
- impregnation of preformed silica gels in protein solution: ex situ method,
- protein incorporation during the silica gel synthesis: in situ method.
The textural properties of silica gels were characterized by nitrogen adsorption and mercury porosimetry. Our results have evidenced that ex situ gels have a funnel-like porous structure (micro, meso and macro) while in situ gel has a microporous structure.
The STI release kinetics was assessed in vitro over 7 days. The comparison of the two immobilization methodologies was particularly interesting, allowing us to correlate the physical properties of our materials with the differences in release kinetics of our model protein. The ex situ technique leads to a rapid release of the protein in less than 24 hours, while the release profile of STI controlled by in situ gel extends over a period longer than 7 days.
Our very encouraging results open new perspectives in the biofunctionalization of matrices and highlight the benefits of working at the interface of the inorganic and organic material sciences and of their applications in life sciences.
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