Master Thesis Online
Environmental parameters influence on lipid accumulation in Scenedesmus obliquus
Desoleil, François 
Promotor(s) :
Jacques, Philippe
Date of defense : 26-Aug-2019 • Permalink : http://hdl.handle.net/2268.2/8345
Details
| Title : | Environmental parameters influence on lipid accumulation in Scenedesmus obliquus |
| Translated title : | [fr] Influence des paramètres environnementaux sur l'accumulation de lipides chez Scenedesmus obliquus |
| Author : | Desoleil, François
|
| Date of defense : | 26-Aug-2019 |
| Advisor(s) : | Jacques, Philippe
|
| Committee's member(s) : | Sindic, Marianne
Fickers, Patrick
Delvigne, Frank
Fauconnier, Marie-Laure
|
| Language : | English |
| Keywords : | [en] microalgae, scenedesmus obliquus, lipid accumulation, nitrogen, temperature, light, pre-treatment, antioxidant |
| Discipline(s) : | Life sciences > Biotechnology |
| Funders : | Lipofabrik Belgium |
| Research unit : | TERRA - Teaching and Research Centre |
| Institution(s) : | Université de Liège, Liège, Belgique |
| Degree: | Master en bioingénieur : chimie et bioindustries, à finalité spécialisée |
| Faculty: | Master thesis of the Gembloux Agro-Bio Tech (GxABT) |
Abstract
[en] Increasingly studied for bio-fuel production, microalgae are able to store large quantities of lipids. The effects of nitrogen starvation, temperature and light stress on growth, lipid accumulation and production in Scenedesmus obliquus S4, a strain supplied by Lipofabrik, were studied. Nitrogen deprivation increased lipid accumulation by a factor of 7, while a daily temperature cycle increased it by a factor of 4. A potential photo-inhibition effect could be highlighted for the S4 strain at 150 µmol/m²/s. The effects of sonication, freeze-drying and freezing on the performance of lipid extraction were also compared, leading to the conclusion that freeze-drying allows the most effective extraction of total lipids.
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Master thesis - GxABT - Francois Desoleil s130121 - 2019.pdf
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