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Gembloux Agro-Bio Tech (GxABT)
Gembloux Agro-Bio Tech (GxABT)
MASTER THESIS
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Etude de la dysfonction mitochondriale dans des modèles d'insuffisance rénale

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Sirjacobs, Damien ULiège
Promotor(s) : Everaert, Nadia ULiège ; Caron, Nathalie
Date of defense : 1-Sep-2020 • Permalink : http://hdl.handle.net/2268.2/10519
Details
Title : Etude de la dysfonction mitochondriale dans des modèles d'insuffisance rénale
Translated title : [en] Study of mitochondrial dysfunction in renal failure
Author : Sirjacobs, Damien ULiège
Date of defense  : 1-Sep-2020
Advisor(s) : Everaert, Nadia ULiège
Caron, Nathalie 
Committee's member(s) : Vandenbol, Micheline ULiège
Schroyen, Martine ULiège
Mosseray, Pauline 
Sindic, Marianne ULiège
Language : English
Keywords : [en] Nephropathy
[en] Aristolochic Acids
[en] Mitochondria
[en] Oxidative Stress
Discipline(s) : Life sciences > Biochemistry, biophysics & molecular biology
Life sciences > Anatomy (cytology, histology, embryology...) & physiology
Funders : Université de Namur
Research unit : Unité de Recherche en Physiologie Moléculaire (URPhyM)
Target public : Researchers
Professionals of domain
Student
Institution(s) : Université de Liège, Liège, Belgique
Université de Namur, Namur, Belgique
Degree: Master en bioingénieur : chimie et bioindustries, à finalité spécialisée
Faculty: Master thesis of the Gembloux Agro-Bio Tech (GxABT)

Abstract

[en] The Aristolochic Acids (AA) Nephropathy (AAN) is a disease discovered in the 90’s in Belgium. It is a tubulointerstitial nephritis defined by two phases: an acute phase called Acute Kidney Injury (AKI) step mainly characterized by oxidative stress and Proximal Tubular Epithelial Cells (PTEC) necrosis followed by a Chronic Kidney Disease (CKD) phase characterized by inflammation and fibrosis. Although the mechanisms of AAN are not fully understood, it has been demonstrated that PTECs are the main target of AA. Furthermore, the involvement of mitochondria in nephropathies is particularly studied as the processes of filtration and secretion require a huge amount of energy. Additionally, many studies pointed the involvement of mitochondria in many metabolic pathways including reactive oxygen species production and detoxification, cell death and inflammation. Previous works in the laboratory highlighted dysfunctions and structural disorders in mitochondria during long term AA-I treatment (from 24 to 72 hours) in HK-2 cells model. Therefore, the present work tried to assess the early events that may have occur during AA-I treatment in order to provide additional information about the timeline of mitochondrial metabolism: If mitochondria trigger HK-2 cells cytotoxicity or their dysfunction is a consequence of cytotoxicity. HK-2 cells were treated with AA-I at concentrations varying from 1 to 25 µM of AA and during timings ranging from 2 to 48 hours. Cell viability and cytotoxicity were studied through MTS and CellToxTM Green assays. The oxidative stress and mitochondrial abundance were respectively assessed through relative PGC-1α, HO-1 and NRF2 mRNA expressions and mtDNA and TOM20 protein abundance.


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Author

  • Sirjacobs, Damien ULiège Université de Liège > Gembloux Agro-Bio Tech

Promotor(s)

Committee's member(s)

  • Vandenbol, Micheline ULiège Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
    ORBi View his publications on ORBi
  • Schroyen, Martine ULiège Université de Liège - ULiège > Département GxABT > Département GxABT
    ORBi View his publications on ORBi
  • Mosseray, Pauline Université de Namur - UNamur > Faculté de Médecine > URPhyM
  • Sindic, Marianne ULiège Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes
    ORBi View his publications on ORBi
  • Total number of views 22
  • Total number of downloads 1










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