Feedback

Faculté de Médecine Vétérinaire
Faculté de Médecine Vétérinaire
MASTER THESIS
VIEW 25 | DOWNLOAD 1

Babesia diagnosis in cheetahs : preliminary analysis comparing PCR tests and microscopic observations of blood smears

Download
Courteille, Romane ULiège
Promotor(s) : Detilleux, Johann ULiège
Date of defense : 21-Jun-2021 • Permalink : http://hdl.handle.net/2268.2/12434
Details
Title : Babesia diagnosis in cheetahs : preliminary analysis comparing PCR tests and microscopic observations of blood smears
Translated title : [fr] DIAGNOSTIC DE BABESIA CHEZ LES GUÉPARDS : UNE ÉTUDE PRÉLIMINAIRE COMPARANT LES TESTS PCR AUX OBSERVATIONS MICROSCOPIQUES DE FROTTIS SANGUINS
Author : Courteille, Romane ULiège
Date of defense  : 21-Jun-2021
Advisor(s) : Detilleux, Johann ULiège
Committee's member(s) : Cabaraux, Jean-Fran�ois ULiège
Vandenheede, Marc ULiège
Language : English
Number of pages : 43
Keywords : [en] Babesia
[en] Cheetahs
[en] PCR
[en] Blood smear
[en] sensitivity
[en] specificity
Discipline(s) : Life sciences > Veterinary medicine & animal health
Research unit : Cheetah Conservation Fund
Target public : Researchers
Professionals of domain
Student
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en médecine vétérinaire
Faculty: Master thesis of the Faculté de Médecine Vétérinaire

Abstract

[en] Cheetahs are on the list of the most endangered feline species of Africa. To have an effective conservation effort it is important to have a global idea of their health, their behaviour, their environment, and their genetic. This multidisciplinary vision is the leitmotiv of the Cheetah Conservation Fund in Namibia. A lot of African felines have blood and tick-borne subclinical diseases, that can with other infection play a co-morbidity role. That’s why it is important to know the prevalence and importance of Babesia infections in cheetahs. For this, we have different methods, with different sensitivities and specificities. With the help of calculations and graphs, we compared 2 methods, the blood smear microscopic examination, which is the gold standard of Babesia diagnosis, and PCR testing of blood samples. We found that the sensitivity was much higher for PCR, especially considering that blood smear examinations have a very variable sensitivity depending on the experience of the person examining the slide. The specificity is at least as good in a PCR than in a blood smear, if not better because it allows identifying the Babesia subspecies with the right primer. In conclusion, PCR is a good alternative to diagnose Babesia infections, especially for cheetahs who generally have low parasitaemia.


File(s)

Document(s)

File
Access COURTEILLE_Romane_TFE_FMV_juin2021_définitif.pdf
Description:
Size: 930.76 kB
Format: Adobe PDF

Author

  • Courteille, Romane ULiège Université de Liège > Master méd. vété.

Promotor(s)

Committee's member(s)

  • Cabaraux, Jean-Fran�ois ULiège Université de Liège - ULiège > Dpt. de gestion vétérinaire des Ressources Animales (DRA) > Ecologie de la santé et des productions animales
    ORBi View his publications on ORBi
  • Vandenheede, Marc ULiège Université de Liège - ULiège > Dpt. de gestion vétérinaire des Ressources Animales (DRA) > Ethologie vétérinaire et bien-être des animaux
    ORBi View his publications on ORBi
  • Total number of views 25
  • Total number of downloads 1










All documents available on MatheO are protected by copyright and subject to the usual rules for fair use.
The University of Liège does not guarantee the scientific quality of these students' works or the accuracy of all the information they contain.