Gembloux Agro-Bio Tech (GxABT)
Gembloux Agro-Bio Tech (GxABT)

Bovine leukemia virus antisense transcription regulates viral and host genome expression

Gouverneur, Lorian ULiège
Promotor(s) : Willems, Luc ULiège
Date of defense : 1-Sep-2021 • Permalink :
Title : Bovine leukemia virus antisense transcription regulates viral and host genome expression
Author : Gouverneur, Lorian ULiège
Date of defense  : 1-Sep-2021
Advisor(s) : Willems, Luc ULiège
Committee's member(s) : Everaert, Nadia ULiège
Joris, Thomas ULiège
Twizere, Jean-Claude ULiège
Massart, Sébastien ULiège
Lassois, Ludivine ULiège
Language : English
Number of pages : 72
Discipline(s) : Life sciences > Biochemistry, biophysics & molecular biology
Life sciences > Biotechnology
Life sciences > Microbiology
Life sciences > Veterinary medicine & animal health
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en bioingénieur : sciences agronomiques, à finalité spécialisée
Faculty: Master thesis of the Gembloux Agro-Bio Tech (GxABT)


[en] Bovine leukemia virus (BLV) is a deltaretrovirus that naturally infects B-cells of cattle, leading to a widespread lymphoproliferative disease termed enzootic bovine leukosis. In infected individuals, the virus is able to escape from the host immune response by viral silencing while maintaining its replicative and oncogenic potential. The recent discovery of BLV-associated antisense transcripts continuously expressed in both non-malignant and leukemic cells have raised the opportunity to unravel key mechanisms behind viral replication and pathogenesis. The objective of this work is to evaluate the importance of BLV antisense transcription in viral expression and replication, as well as its influence on host genome expression. Data show that mutation of two Sp-1 binding sites inhibits the antisense transcriptional activity of the 3' long terminal repeat (LTR) minimal antisense promoter in vitro. The mutation carried out in the U5 region on a full-length LTR also induces a reduction of antisense transcription, but consequently increases sense transcriptional activity. In the context of a mutated full-length provirus, viral sense and antisense expression are dysregulated in infected animals while proviral loads decrease drastically. Furthermore, the expression of host genes, especially TPPP and HMCN1, is influenced by altered antisense transcriptional activity. Antisense transcription is therefore able to control viral replication by mediating sense transcription and interferes with host genome expression.



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  • Gouverneur, Lorian ULiège Université de Liège > Gembloux Agro-Bio Tech


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