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Gembloux Agro-Bio Tech (GxABT)
Gembloux Agro-Bio Tech (GxABT)
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Evaluation of pesticides degradation by microorganisms in the soil of a new cropping system : Isolation and characterization of pesticide-degrading strains of soil microorganisms

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Guillaume, Benjamin ULiège
Promotor(s) : Degré, Aurore ULiège ; De Clerck, Caroline ULiège
Date of defense : 1-Sep-2021 • Permalink : http://hdl.handle.net/2268.2/13177
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Title : Evaluation of pesticides degradation by microorganisms in the soil of a new cropping system : Isolation and characterization of pesticide-degrading strains of soil microorganisms
Author : Guillaume, Benjamin ULiège
Date of defense  : 1-Sep-2021
Advisor(s) : Degré, Aurore ULiège
De Clerck, Caroline ULiège
Committee's member(s) : Massart, Sébastien ULiège
Dincher, Marie ULiège
Pirlot, Clémence ULiège
Pigeon, Olivier 
Charles, Catherine ULiège
Language : English
Number of pages : 89
Keywords : [en] Biodegradation
[en] Metamitron
[en] Metazachlor
[en] Bentazon
[en] Metolachlor
[en] Chlortoluron
[en] Soil column
[en] Phenol oxidase
[en] Sterilisation
Discipline(s) : Life sciences > Microbiology
Life sciences > Agriculture & agronomy
Life sciences > Environmental sciences & ecology
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en bioingénieur : sciences et technologies de l'environnement, à finalité spécialisée
Faculty: Master thesis of the Gembloux Agro-Bio Tech (GxABT)

Abstract

[fr] Among the process that determine the fate of pesticides in soils, microbial degradation is recognized as one of the most important. Biodegradation of pesticides seems to be achieved through the contribution of a few key species that exhibit degradation capabilities. However, in field conditions the real effect of these key species on the fate of pesticides is still poorly understood. We attempted to isolate and identify some of them capable of degrading metamitron, metazachlor, bentazon, s-metolachlor and chlortoluron after selective enrichmeent cultures in minimal medium. Their degradation capacities were evaluated in a minimal liquid medium with pesticide as the only source of carbon and nitrogen. The feasibility of assessing the degradation behaviour of degrading strains on undisturbed soil columns was performed. A special focus was made on the effect of autoclaving on soil properties that might control the fate of pesticides. In total, 85 pesticide-resistant cultures were isolated. 27 of them showed significant enzyme capacity with ABTS as substrate which may indicate their ability to degrade phenolic compounds. Three isolated strains (SM 12, SM 2 and SM 14) were suggested to be able to degrade metolachlor. Three other strains (MET 8, MET 17 and MET 12) were proposed to be able to degrade metamitron. One strain (MZ 3) was potentially able to degrade metazachlor. The soil column study revealed for the first time that autoclaving may influence the soil hydraulic conductivity by affecting the soil structure. This can lead to inaccurate assessments of pesticide fate in experiments conducted under abiotic soil columns and inoculated with degrading strains.


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Author

  • Guillaume, Benjamin ULiège Université de Liège > Gembloux Agro-Bio Tech

Promotor(s)

Committee's member(s)

  • Massart, Sébastien ULiège Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs
    ORBi View his publications on ORBi
  • Dincher, Marie ULiège Université de Liège - ULiège > Département GxABT > Echanges Eau - Sol - Plantes
    ORBi View his publications on ORBi
  • Pirlot, Clémence ULiège Université de Liège - ULiège >
    ORBi View his publications on ORBi
  • Pigeon, Olivier Centre wallon de Recherches agronomiques (CRA-W) > Département connaissance et valorisation des produits > Unité produits de protection, de contrôle et résidus
  • Charles, Catherine ULiège Université de Liège - ULiège > Département GxABT > Biosystems Dynamics and Exchanges
    ORBi View his publications on ORBi
  • Total number of views 79
  • Total number of downloads 547










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