Thesis, COLLÉGIALITÉ

Abstract

Nitric oxide synthase expressing neurons (nNOS) located in the ventrolateral part of the ventromedial hypothalamus (VMHvl) are known to be targeted by kisspeptin projections coming from the anteroventral periventricular nucleus (AVPV) and to be crucial for the expression of the effects of kisspeptin on female sexual behavior Moreover, nNOS knockout male mice show impaired sexual and aggressive behavior. Interestingly, VMHvl progesterone (PR) and estrogen receptor alpha (Eralpha) expressing neurons are known to regulate both aggressive and sexual behavior in mice, regardless of sex. We hypothesize that the expression of nNOS in the VMHvl might overlap with Eralpha and PR expression. Thus, a role of VMHvl nNOS neurons on male and female aggression and sexual behavior is likely. The aim of our study is to evaluate how the deletion of nNOS positive neurons in the VMHvl affects, social and sexual motivation as well as sexual and aggressive behaviors in both sexes. In order to achieve that, we will use techniques such as viral infusions, stereotaxic surgery, behavioral assessment, and immunohistochemistry.