Gembloux Agro-Bio Tech (GxABT)
Gembloux Agro-Bio Tech (GxABT)

Establishment of a cell line for the production of vaccine against bovine leukaemia virus

Jouant, Thomas ULiège
Promotor(s) : Willems, Luc ULiège ; Jacques, Jean-Rock ULiège
Date of defense : 29-Aug-2022 • Permalink :
Title : Establishment of a cell line for the production of vaccine against bovine leukaemia virus
Translated title : [fr] Etablissement d'une lignée cellulaire pour la production d'un vaccin contre le virus de la leucémie bovine
Author : Jouant, Thomas ULiège
Date of defense  : 29-Aug-2022
Advisor(s) : Willems, Luc ULiège
Jacques, Jean-Rock ULiège
Committee's member(s) : Schroyen, Martine ULiège
Sindic, Marianne ULiège
Twizere, Jean-Claude ULiège
Deleu, Magali ULiège
Language : English
Number of pages : 86
Keywords : [en] Bovine leukaemia virus
[en] vaccine
Discipline(s) : Life sciences > Biochemistry, biophysics & molecular biology
Life sciences > Veterinary medicine & animal health
Life sciences > Biotechnology
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en bioingénieur : chimie et bioindustries, à finalité spécialisée
Faculty: Master thesis of the Gembloux Agro-Bio Tech (GxABT)


[en] Bovine Leukaemia Virus (BLV) is a retrovirus that infect cattle, yak, water buffalo and zebu. The most common manifestation of the virus is an accumulation of infected B lymphocytes called persistent lymphocytosis (PL). This virus is present all over the world except in western Europe where a large campaign of testing and euthanasia of infected animals has been conducted. BLV economic impact is estimated at US$525 million due to a loss of milk production but also to a shorter animal lifespan. Preventive actions implemented to limit the spread of the virus often fail due to a lack of commitment to the programme. Therefore, a vaccine is urgently needed to facilitate herd management and disease control. A safe and effective vaccine based on an attenuated BLV strain (pBLV6073DX) has recently been developed. However, the current inoculation technique based on purified DNA does not allow for large-scale vaccination. In this context, the objective of this master thesis is to obtain a cell line expressing the vaccine strain in order to consider its larger-scale production. For this purpose, Vero cells were either transfected with the proviral insert or transduced with viral particles. After sorting with a flow cytometer and PCR-based clone selection, a cell line having stably integrated the vaccine sequence was isolated. Inoculation of sheep with this cell line effectively transmitted the vaccine strain, providing the proof-of-concept of the approach.



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  • Jouant, Thomas ULiège Université de Liège > Gembloux Agro-Bio Tech


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