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Gembloux Agro-Bio Tech (GxABT)
Gembloux Agro-Bio Tech (GxABT)
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Etude du potentiel probiotique des microorganismes de diverses matrices biologiques pour des applications en alimentation animale

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Yao, Ange Olivier Parfait ULiège
Promotor(s) : Jacques, Philippe ULiège ; Razafindralambo, Hary ULiège
Date of defense : 23-Jun-2023 • Permalink : http://hdl.handle.net/2268.2/17866
Details
Title : Etude du potentiel probiotique des microorganismes de diverses matrices biologiques pour des applications en alimentation animale
Translated title : [en] STUDY OF THE PROBIOTIC POTENTIAL OF MICROORGANISMS IN VARIOUS BIOLOGICAL MATRICES FOR ANIMAL FEED APPLICATIONS.
Author : Yao, Ange Olivier Parfait ULiège
Date of defense  : 23-Jun-2023
Advisor(s) : Jacques, Philippe ULiège
Razafindralambo, Hary ULiège
Committee's member(s) : Purcaro, Giorgia ULiège
Daube, Georges ULiège
Drider, Jamel 
Malumba Kamba, Paul ULiège
Alabi, Taofic ULiège
Fickers, Patrick ULiège
Language : French
Keywords : [fr] probiotic
[fr] lactic acid bacteria
[fr] adhesion
[fr] antagonism
[fr] MALDI-TOF SM
[fr] 16S DNA
Discipline(s) : Life sciences > Biotechnology
Name of the research project : URBANE-PROJECT
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en bioingénieur : chimie et bioindustries, à finalité spécialisée
Faculty: Master thesis of the Gembloux Agro-Bio Tech (GxABT)

Abstract

[fr] Probiotics are microorganisms of varied origins, capable of positively impacting intestinal flora. The probiotic potential of microbial strains isolated from biological matrices from Morocco was studied based on several criteria. This included antibiotic resistance, the ability to adhere to solvents, the ability to produce antimicrobial substances, and the ability to colonize the gastrointestinal tract. Molecular (16S DNA) and analytical (MALDI-ToF-MS) techniques have been used to identify several microorganisms. The probiotic properties of five of them (Lactobacillus plantarum from sourdough, Leuconostoc pseudomesenteroides and Leuconostoc mesenteroides isolated from fresh goat's cheese, Enterococcus durans and Lactobacillus casei isolated from fermented milk) were characterized including their growth in MRS broth, their viability at pH 2.5 and 6.5 in the presence of bile salts (1%), their surface hydrophobicity, their ability to autoaggregate, their thermogravimetric decomposition pattern and their ability to express antagonistic activity towards certain pathogens. The results of antagonism tests showed that isolated Lactobacillus plantarum and Leuconostoc mesenteroides strains inhibited the growth of Bacillus cereus, Staphylococcus epidermidis and Escherichia coli pathogens, while Enterococcus durans and Lactobacillus casei strains were effective against B. cereus and E. coli. Antibiotic susceptibility results were satisfactory for most strains, except for Enterococcus durans, which showed resistance to each of the antibiotics tested. This strain was, however, the only one to grow at pH 2.5 in the presence of 1% bile salts, giving it the ability to survive in gastrointestinal conditions. The ability of strains to produce emulsifying substances was studied by determining the emulsion index (expressed as a percentage) of culture supernatants mixed within toluene. The results showed that Leuconostoc pseudomesenteroides culture supernatants had the highest emulsion-forming capacity (81.72%). However, the supernatant of Leuconostoc mesenteroides showed the best emulsifying stability which only went from 8.7% at 0h to 8.6% after 24h. Overall, all the bacteria used have a good autoaggregation capacity (over 90%). The surface hydrophobicity properties expressed as a percentage represent the ability of the bacterial cells studied to grow on an immiscible substrate. These percentages vary from species to species and are 36.82%, 28.18%, 39.23%, and 26.72% respectively for Lactobacillus plantarum, Leuconostoc pseudomesenteroides, Enterococcus durans, and Lactobacillus casei. These last two parameters were the main surface properties evaluated in this study. All these observations clearly show that the matrices analyzed obviously contain potential probiotic candidates.


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Author

  • Yao, Ange Olivier Parfait ULiège Université de Liège > Gembloux Agro-Bio Tech

Promotor(s)

Committee's member(s)

  • Purcaro, Giorgia ULiège Université de Liège - ULiège > Département GxABT > CSFES
    ORBi View his publications on ORBi
  • Daube, Georges ULiège Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
    ORBi View his publications on ORBi
  • Drider, Jamel Université de Lille > Génie Biologique de l'Institut Universitaire de Technologie (IUT-U Lille) > Enseignant chercheur
  • Malumba Kamba, Paul ULiège Université de Liège - ULiège > Département GxABT > Technologie Alimentaire (TA)
    ORBi View his publications on ORBi
  • Alabi, Taofic ULiège Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs
    ORBi View his publications on ORBi
  • Fickers, Patrick ULiège Université de Liège - ULiège > Département GxABT > Microbial technologies
    ORBi View his publications on ORBi
  • Total number of views 132
  • Total number of downloads 347










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