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Gembloux Agro-Bio Tech (GxABT)
Gembloux Agro-Bio Tech (GxABT)
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Virome analysis of Lolium perenne L in two ecosystems ( pasture and grassland) in Belgium

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Schrurs, Ségolène ULiège
Promotor(s) : Massart, Sébastien ULiège ; Selmi, Ilhem ULiège
Date of defense : 25-Aug-2023 • Permalink : http://hdl.handle.net/2268.2/18302
Details
Title : Virome analysis of Lolium perenne L in two ecosystems ( pasture and grassland) in Belgium
Author : Schrurs, Ségolène ULiège
Date of defense  : 25-Aug-2023
Advisor(s) : Massart, Sébastien ULiège
Selmi, Ilhem ULiège
Committee's member(s) : Jijakli, Haissam ULiège
De Clerck, Caroline ULiège
Maclot, Francois 
Massart, Sébastien ULiège
Selmi, Ilhem ULiège
Purcaro, Giorgia ULiège
Danthine, Sabine ULiège
Language : English
Keywords : [en] Poaceae
[en] Lolium perenne
[en] pasture
[en] grassland
Discipline(s) : Life sciences > Biochemistry, biophysics & molecular biology
Life sciences > Biotechnology
Research unit : Department de Phytopathologie Gembloux Agro Bio Tech
Target public : Professionals of domain
Student
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en bioingénieur : chimie et bioindustries, à finalité spécialisée
Faculty: Master thesis of the Gembloux Agro-Bio Tech (GxABT)

Abstract

[en] In order to analyze the virome of Lolium perenne in Belgium, 78 samples were collected across two low-maintained ecosystems such as pastures and grasslands. The Virion-Associated-Nucleic Acids library preparation and High Throughput Sequencing enabled the detection of 20 viral species. Totiviridae and Partitiviridae species dominating the grassland ecosystem and Solemoviridae and Tombusviridae members were for the pasture field. RT-PCR was used to validate HTS detection for ryegrass mosaic virus , lolium latent virus and BYDV-PAV or more recently renamed luteovirus pavhordei). The Peta scale database provided by serratus.io was searched and expanded knowledge regarding novel liege poaecae viruses. Presence of aphid-transmitted and persistent viruses stress vector’s role in virus spread and the need for broader wild virus reservoir studies


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  • Schrurs, Ségolène ULiège Université de Liège > Gembloux Agro-Bio Tech

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