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Gembloux Agro-Bio Tech (GxABT)
Gembloux Agro-Bio Tech (GxABT)
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Enhancing cellulose accessibility in oil palm empty fruit bunches (OPEFB) through optimized maleic acid pretreatment for improved glucose-to-ethanol bioconversion

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Postiaux, Annaëlle ULiège
Promotor(s) : Richel, Aurore ULiège ; Ogino, Chiaki
Date of defense : 22-Jan-2024 • Permalink : http://hdl.handle.net/2268.2/19430
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Title : Enhancing cellulose accessibility in oil palm empty fruit bunches (OPEFB) through optimized maleic acid pretreatment for improved glucose-to-ethanol bioconversion
Translated title : [fr] AMÉLIORATION DE L'ACCESSIBILITÉ DE LA CELLULOSE DANS LES GRAPPES DE FRUITS VIDES DE PALMIERS À HUILE PAR UN PRÉTRAITEMENT OPTIMISÉ À L'ACIDE MALÉIQUE POUR UNE MEILLEURE BIOCONVERSION DU GLUCOSE EN ÉTHANOL
Author : Postiaux, Annaëlle ULiège
Date of defense  : 22-Jan-2024
Advisor(s) : Richel, Aurore ULiège
Ogino, Chiaki 
Committee's member(s) : Fauconnier, Marie-Laure ULiège
Jacquet, Nicolas ULiège
Genva, Manon ULiège
Kahar, Prihardi 
Putra, Filemon 
Purcaro, Giorgia ULiège
Richel, Aurore ULiège
Ogino, Chiaki 
Language : English
Number of pages : 89
Keywords : [en] Biorefinery
[en] Lignocellulose
[en] Pretreatment
[en] Organosolv
[en] Maleic acid
[en] Cellulose
[en] Accessibility
[en] Saccharification
[en] Glucose
[en] Fermentation
[en] Saccharomyces cerevisiae F118
[en] Ethanol
[en] FTIR
[en] HPLC
[en] XRD
Discipline(s) : Engineering, computing & technology > Chemical engineering
Life sciences > Biotechnology
Research unit : Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, 657-8501, Japan.
Target public : Researchers
Professionals of domain
Student
General public
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en bioingénieur : chimie et bioindustries, à finalité spécialisée
Faculty: Master thesis of the Gembloux Agro-Bio Tech (GxABT)

Abstract

[en] Pretreatment of lignocellulosic biomass remains a limiting stage in sustainable energy production. Studies are still needed to understand the principles and find an efficient process for converting lignocellulosic biomass into bioethanol. This study aims to improve the accessibility of the cellulose contained in oil palm empty fruit bunch by improving pretreatment parameters. The impact of maleic acid concentration, pretreatment temperature and pretreatment time on each stage of the process is investigated. First the biomass is pretreated, then enzymatic saccharification is performed on the remaining solid, and finally fermentation of the resulting hydrolysate is carried out using Saccharomyces cerevisiae F118, an engineered yeast.
Pretreatment with 75% maleic acid for 60 minutes at 180°C resulted in more efficient removal of lignin and hemicellulose, and therefore greater accessibility of the cellulose. However, the cellulose obtained at the end of pretreatment has a crystallinity of 74.8%, which slows down enzymatic attack during saccharification. Biomass pretreated with maleic acid at a concentration of 50% for 60 minutes at 180°C gives a higher glucose yield of 77.1%. Finally, greater ethanol production is obtained with the hydrolysate obtained from enzymatic saccharification of biomass pretreated with maleic acid at 1% concentration for 60 minutes at 180°C. Ethanol yield is affected by the presence of maleic acid adsorbed on the solid matrix.


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Author

  • Postiaux, Annaëlle ULiège Université de Liège > Gembloux Agro-Bio Tech

Promotor(s)

Committee's member(s)

  • Fauconnier, Marie-Laure ULiège Université de Liège - ULiège > Département GxABT > CSFES
    ORBi View his publications on ORBi
  • Jacquet, Nicolas ULiège Université de Liège - ULiège > Département GxABT > Technologie Alimentaire (TA)
    ORBi View his publications on ORBi
  • Genva, Manon ULiège Université de Liège - ULiège > Département GxABT > CSFES
    ORBi View his publications on ORBi
  • Kahar, Prihardi
  • Putra, Filemon
  • Purcaro, Giorgia ULiège Université de Liège - ULiège > Département GxABT > CSFES
    ORBi View his publications on ORBi
  • Richel, Aurore ULiège Université de Liège - ULiège > Département GxABT > CSFES
    ORBi View his publications on ORBi
  • Ogino, Chiaki Kobe University, 1-1 Rokkodai-cho, Nada-ku, Hyogo, 657-8501, Japan > Department of Chemical Science and Engineering > Graduate School of Engineering
  • Total number of views 23
  • Total number of downloads 3










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