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Gembloux Agro-Bio Tech (GxABT)
Gembloux Agro-Bio Tech (GxABT)
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Bovine leukaemia virus antisense transcription is required for viral replication

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Joris, Thomas ULiège
Promotor(s) : Willems, Luc ULiège
Date of defense : 26-Aug-2019 • Permalink : http://hdl.handle.net/2268.2/7882
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Title : Bovine leukaemia virus antisense transcription is required for viral replication
Translated title : [fr] La transcription antisens du virus de la leucémie bovine est nécessaire pour la réplication virale
Author : Joris, Thomas ULiège
Date of defense  : 26-Aug-2019
Advisor(s) : Willems, Luc ULiège
Committee's member(s) : Everaert, Nadia ULiège
Sindic, Marianne ULiège
Vandenbol, Micheline ULiège
Safari, Roghaiyeh ULiège
Language : English
Discipline(s) : Life sciences > Biochemistry, biophysics & molecular biology
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en bioingénieur : chimie et bioindustries, à finalité spécialisée
Faculty: Master thesis of the Gembloux Agro-Bio Tech (GxABT)

Abstract

[en] The bovine leukaemia virus (BLV) is a deltaretrovirus naturally infecting B-cells in cattle, which can lead in 5% of cases to an aggressive condition called enzootic bovine leukosis. The recent discovery of antisense transcripts, continuously expressed in both asymptomatic and leukaemic cells, opens new perspectives for understanding viral replication or pathogenesis. The objective of this work is to evaluate the importance of BLV antisense transcription in viral replication. Results show that a two-nucleotides mutation of the SP1 binding site drastically inhibits the transcriptional activity of the minimal 3’LTR promoter. In the context of a full-length provirus, this mutation induces a significant decrease of antisense transcripts in cell culture, and in a drastic reduction of proviral loads in infected animals. Antisense transcription thus plays a crucial role in viral replication.


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Author

  • Joris, Thomas ULiège Université de Liège > Gembloux Agro-Bio Tech

Promotor(s)

Committee's member(s)

  • Everaert, Nadia ULiège Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions animales et nutrition
    ORBi View his publications on ORBi
  • Sindic, Marianne ULiège Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
    ORBi View his publications on ORBi
  • Vandenbol, Micheline ULiège Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
    ORBi View his publications on ORBi
  • Safari, Roghaiyeh ULiège Université de Liège - ULiège > Cancer-Cellular and Molecular Epigenetics
    ORBi View his publications on ORBi
  • Total number of views 35
  • Total number of downloads 1










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