Evaluation of the neovascularisation of 3D-printed hydroxyapatite implants in rats
Desonniaux, Marie
Promotor(s) : Cobraiville, Elisabeth
Date of defense : 6-Sep-2021 • Permalink : http://hdl.handle.net/2268.2/12878
Details
Title : | Evaluation of the neovascularisation of 3D-printed hydroxyapatite implants in rats |
Translated title : | [fr] évaluation de la néovascularisation des implant imprimé en 3D chez le rat |
Author : | Desonniaux, Marie |
Date of defense : | 6-Sep-2021 |
Advisor(s) : | Cobraiville, Elisabeth |
Committee's member(s) : | Nolens, Grégory
RONSMANS, Christophe Stordeur, Philippe |
Language : | English |
Number of pages : | 59 |
Discipline(s) : | Life sciences > Biotechnology |
Institution(s) : | Université de Liège, Liège, Belgique |
Degree: | Master en sciences biomédicales, à finalité approfondie |
Faculty: | Master thesis of the Faculté de Médecine |
Abstract
[en] Introduction: Maxillo-facial defects are a commonly encountered problem that cranio-facial and plastic surgeons have to face in hospital practice. It can have either a pathologic or a non-pathologic origin. The current gold standard for bone replacement for in those defects is an autograft from a fibula free flap. However, the current gold standard has limitations and synthetic bone replacement overcoming those limitations have known an expansion in the recent years.
Objective: Two main objectives were pursued during this master thesis. The first one was to develop and evaluate the design of the sub-scapular implant as well as the surgical implantation protocol. The second was to develop and evaluate the imaging protocols to visualise the neovascularisation inside the implant.
Methods: First a cadaver test was realised to test and evaluate the design of the implant as well as the surgical implantation technique. Secondly, a pilot study was realised in a small group of 5 rats. During this pilot study, the surgical technique was adapted and the vascularisation was evaluated by micro-CT. Both in-vivo and ex-vivo micro-CT were used.
Results: The design medium rectangular L was the design the most fitting regarding size and implan- tation incision closure. The tail catheter injection technique was chosen over the needle injection. The in-vivo micro-CT show vascularisation only on the outside of implant. The pre-test ex-vivo micro-CT shown vascularisation inside organs.
Conclusion: The medium rectangular L design was chosen to be implanted in the rats of the pilot study. The placement of the medium rectangular L at the interscapular area in rats with the chosen protocol was successful. The vascularisation surrounding the implant can be seen with the in-vivo micro-CT. However, the neovascularisation inside the implant could not be seen with that imaging method.
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