Feedback

Faculté des Sciences
Faculté des Sciences
MASTER THESIS
VIEW 38 | DOWNLOAD 2

Molecular insights into fetal alcohol syndrome : A cerebral cortex perspective

Download
Boutsen, Anaïs ULiège
Promotor(s) : Nguyen, Laurent ULiège ; Hanikenne, Marc ULiège
Date of defense : 25-Jan-2022 • Permalink : http://hdl.handle.net/2268.2/13901
Details
Title : Molecular insights into fetal alcohol syndrome : A cerebral cortex perspective
Author : Boutsen, Anaïs ULiège
Date of defense  : 25-Jan-2022
Advisor(s) : Nguyen, Laurent ULiège
Hanikenne, Marc ULiège
Committee's member(s) : Malgrange, Brigitte ULiège
Vandenbosch, Renaud ULiège
Marichal, Thomas ULiège
Language : English
Keywords : [en] FASD
[en] scRNA-seq
Discipline(s) : Human health sciences > Multidisciplinary, general & others
Research unit : Nguyen Laboratory
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en bioinformatique et modélisation, à finalité approfondie
Faculty: Master thesis of the Faculté des Sciences

Abstract

[en] Maternal alcohol consumption during pregnancy can trigger a wide range of developmental, neurobehavioral, neurobiological, and physiological impairments. The spectrum of alcohol’s teratogenic diseases is known as fetal alcohol spectrum disorder (FASD). Among the different mechanisms impacted on the central nervous system (CNS), one of the most important abnormalities appears to be neuronal cell migration. Furthermore, one of the migration processes impacted is the multipolar to bipolar step of the inside out neuronal migration.

During my master thesis, we studied the consequences of alcohol upon its prenatal exposure in the somatosensory cortex, especially migrating neurons. The maternal alcohol consumption was emulated with the “drinking in the dark” mouse paradigm. Single-cell RNA sequencing (scRNA-seq) was used to analyze the somatosensory cortex of E17 mouse embryo exposed to alcohol during pregnancy.

A scRNA-seq analysis pipeline adjusted especially for this experiment showed the presence of 11 cell populations: interneurons, pericytes, endothelial cells, microglia, apical and intermediate progenitors, astrocytes, oligodendrocytes progenitors cells, cajal retzius cells, newborn projection migrating neurons and cortical projection neurons. Alcohol did not impact the proportion and the fate of the cells. Differentially expressed genes were found and used to study enriched pathways and regulation networks. This resulted in pathways’ discovery such as genes involved in the regionalization of the brain.


File(s)

Document(s)

File
Access s205430Boutsen2021.pdf
Description:
Size: 14.95 MB
Format: Adobe PDF

Annexe(s)

File
Access s205430Boutsen2021Annexes.pdf
Description:
Size: 6.16 MB
Format: Adobe PDF

Author

  • Boutsen, Anaïs ULiège Université de Liège > Master bioinf. & mod., à fin.

Promotor(s)

Committee's member(s)

  • Malgrange, Brigitte ULiège Université de Liège - ULiège > Département des sciences cliniques > GIGA Stem Cells - Developmental Neurobiology
    ORBi View his publications on ORBi
  • Vandenbosch, Renaud ULiège Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
    ORBi View his publications on ORBi
  • Marichal, Thomas ULiège Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > GIGA
    ORBi View his publications on ORBi
  • Total number of views 38
  • Total number of downloads 2










All documents available on MatheO are protected by copyright and subject to the usual rules for fair use.
The University of Liège does not guarantee the scientific quality of these students' works or the accuracy of all the information they contain.