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Faculté de Médecine
Faculté de Médecine
MASTER THESIS
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Thesis, COLLÉGIALITÉ

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Stepniak, Marty ULiège
Promotor(s) : Volders, Paul
Date of defense : 5-Jul-2022 • Permalink : http://hdl.handle.net/2268.2/14846
Details
Title : Thesis, COLLÉGIALITÉ
Translated title : [en] Generation of hiPSC lines by PBMCs reprogramming and differentiation into contractile cardiomyocytes.
Author : Stepniak, Marty ULiège
Date of defense  : 5-Jul-2022
Advisor(s) : Volders, Paul 
Committee's member(s) : Cataldo, Didier ULiège
Detry, Olivier ULiège
Quesada Calvo, Florence 
Language : French
Number of pages : 72
Keywords : [fr] cellules souches / cardiomyocytes / hiPSC/ Maastricht University / Uliege/ culture cellulaire
Discipline(s) : Human health sciences > Laboratory medicine & medical technology
Funders : Maastricht University / Uliège
Research unit : CARIM center / Maastricht University
Name of the research project : Stem cell reprogramming and induced cardiomyocytes.
Target public : Researchers
Professionals of domain
Student
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en sciences biomédicales, à finalité approfondie
Faculty: Master thesis of the Faculté de Médecine

Abstract

[en] Stem cells have for all time, and in the general consciousness, held out the promise of cell
regeneration, treatment of genetic diseases, grafts of new functional tissues, etc. A global
solution to very diverse pathologies, as the potential of these cells seems unlimited.
Today, these very special cells have indeed allowed significant advances in clinical and
translational medicine, on a small scale. It is therefore fundamental research that has finally
been able to take advantage of the almost unlimited differentiation potential of these cells,
making it possible to characterize pathological or cellular processes that were previously
unanswered, while waiting for a new model.
In this context, this thesis will focus on the reprogramming of somatic blood cells from patients
into induced pluripotent stem cells (hiPSC) and their differentiation into cardiac contractile
cells.
The objectives are multiple: The development and optimization of a protocol for the
reprogramming of blood cells into pluripotent cells and their subsequent differentiation into
cardiomyocytes. The characterization of cells at key stages of their development: from stem
cells to cardiomyocytes. And finally, in the short term, the electrophysiological analysis of the
induced cardiomyocytes to characterize the impact of specific inherited genetic mutations
carried by patients with a ventricular arrhythmia phenotype.


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Author

  • Stepniak, Marty ULiège Université de Liège > Master sc. bioméd., à fin.

Promotor(s)

  • Volders, Paul Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Committee's member(s)

  • Cataldo, Didier ULiège Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
    ORBi View his publications on ORBi
  • Detry, Olivier ULiège Université de Liège - ULiège > Département des sciences cliniques > Pathologie chirurgicale abdominale et endocrinienne
    ORBi View his publications on ORBi
  • Quesada Calvo, Florence HEPL (Haute école province de Liège) > Sciences Biomédicales
  • Total number of views 37
  • Total number of downloads 70










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