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Faculté des Sciences
Faculté des Sciences
MASTER THESIS
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Unveiling novel streptomyces developmental signal mediated by ABC transporters

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Jadot, Cédric ULiège
Promotor(s) : Rigali, Sébastien ULiège ; Kerff, Frédéric ULiège
Date of defense : 5-Sep-2022 • Permalink : http://hdl.handle.net/2268.2/16525
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Title : Unveiling novel streptomyces developmental signal mediated by ABC transporters
Author : Jadot, Cédric ULiège
Date of defense  : 5-Sep-2022
Advisor(s) : Rigali, Sébastien ULiège
Kerff, Frédéric ULiège
Committee's member(s) : Dumoulin, Mireille ULiège
Deflandre, Benoit ULiège
Ongena, Marc ULiège
Language : English
Number of pages : 69
Keywords : [fr] Streptomyces
[fr] ABC transporters
[fr] metabolic pathway
Discipline(s) : Life sciences > Biochemistry, biophysics & molecular biology
Target public : Researchers
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General public
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Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en biochimie et biologie moléculaire et cellulaire, à finalité approfondie
Faculty: Master thesis of the Faculté des Sciences

Abstract

[en] Streptomyces spp are gram-positive bacteria known for their ecological diversity, their particular development cycle and their ability to produce numerous bioactive molecules. This ecological and therefore genetic diversity requires the ability of its organisms to be able to use a wide variety of carbon sources serving as signals for the production of enzymes such as glycosyl hydrolases or secondary metabolites. However, at present, the regulatory pathways related to the use of these carbon sources are still poorly known since only 6 systems have been studied to date and it is estimated between 10 and 40, the number of systems still unknown. This is why this work aims to complete and expand knowledge on these systems. To do this, we studied the ability of the binding protein MalE to bind malto-oligosaccharides via different methods (intrinsic fluorescence, affinity column and crystallography), we were also interested in the transcriptional regulator MalR associated with this transport system. And during the second part of this work, we sought to discover which ATP Binding Cassette-transporter (ABC-transporter) was involved in the transport of manno-oligosaccharides by testing a candidate binding protein, ManE2.
The results obtained during this work showed the ability of MalE to bind maltose and comparisons with other proteins of known structures suggest an ability to bind maltotriose as well as maltotetraose. We were also able to make progress on the production and purification of MalR. And concerning ManE2 we were able to demonstrate by intrinsic fluorescence experiments its ability to bind mannobiose, mannotiose as well as mannotetraose. This study thus makes it possible to complete the knowledge concerning the use of malto-oligosaccharides as well as to identify a new ABC-transporter for manno-oligosaccharides. Finally, this work allowed the use of various methods allowing to study the interactions proteins ligands and being able to be reused for future studies.


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  • Jadot, Cédric ULiège Université de Liège > Master bioch. & biol. mol. & cel., à fin.

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