Faculté de Médecine
Faculté de Médecine


Lu, Junyan ULiège
Promotor(s) : Vandeweerd, Jean-Michel
Date of defense : 6-Feb-2023 • Permalink :
Title : Thesis, COLLÉGIALITÉ
Author : Lu, Junyan ULiège
Date of defense  : 6-Feb-2023
Advisor(s) : Vandeweerd, Jean-Michel 
Committee's member(s) : Antoine, Nadine 
Nicaise, Charles 
De Meeus, Constance 
Gustin, Pascal 
Language : English
Keywords : [en] infrapatellar fat pad, traumatic osteoarthritis, age-related osteoarthritis, regenerative medicine, ovine model, cruciate ligament transection (ACLT), partial medial meniscectomy (pMMx)
Discipline(s) : Life sciences > Veterinary medicine & animal health
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en sciences biomédicales, à finalité approfondie
Faculty: Master thesis of the Faculté de Médecine


[en] Background: Among the many structures of the knee joint, the function of IFP remains
unclear. It seems to be involved in biomechanics, inflammatory response, blood
supplement for other knee structures, proprioception, and it is also an potential reservoir
of stem cells. Osteoarthritis (OA) has developed into a disease with high incidence. IFP
has been thought to be more and more considered in OA pathobiology. At present, there
is still no perfect treatments for OA, and both conservative treatment and surgery still
need to be improved. Regenerative therapy is popular in musculoskeletal therapy and
has been used by veterinarians for tendon repair. But we still don’t know how does it
work on IFP. So this paper aimed to conduct a systematic review on PRP in regenerative
therapy in house tendon, and to access the pathobiology of IFP in OA.
Methods: Firstly, a systematic review is conducted to provide basic knowledge on
whether regenerative medicine can be applied to OA treatment. For the experimental
part, histological staining was used to assess pathobiology of IFP in traumatic OA and
age-related OA. Traumatic OA was induced by anterior cruciate ligament transection
and partial meniscectomy (ACLT and pMMX). Haematoxylin Eosine Safran (HES) was
used to assess the morphology of the fat pad, and score intima, fibrosis, vascularization,
and inflammatory infiltration. Toluidine blue O - Fast Green (TBOFG) staining was
used to characterize the presence of proteoglycans in the interstitial area of the fat pad.
Picrosirius red-weigert’s hematoxylin (PSR) staining enabled to assess collagen content
and organization.
Results: There is a lot of literature on regenerative medicine for horse tendon injuries,
however, literature lacks definitive evidence of efficiency for regenerative medicine.
There is on statistical difference on the score of Intimal hyperplasia, Proteoglycans,
Sub-intimal fibrosis and Vascularity between young and eldly sheep. While in traumatic
OA, they rise early in the disease (4w-6w) and fall down at 28w. The same trend was
observed for the opposite leg, although there was no statistical difference. At 28w, the
score of PSR (| RF0 - RF30 |) was the highest compared with both control and early
stage of the disease (4w-6w). Cartilages were found in some of the traumatic OA they
were not present in in uninjured sheep.
Conclusions: Surgery or trauma can cause acute stress of IFP and may be a risk factor
for chondrodifferentiation of IFP. This stress degrades over time and the collagen
becomes parallel in the late stage. ACLT and pMMX-induced traumatic OA did not
induce significant compensatory or decompensated response in contralateral leg. The
natural incidence of pathological changes of OA in IFP is low in elderly sheep.



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  • Lu, Junyan ULiège Université de Liège > Master sc. bioméd., à fin.


Committee's member(s)

  • Antoine, Nadine
  • Nicaise, Charles
  • De Meeus, Constance
  • Gustin, Pascal
  • Total number of views 20
  • Total number of downloads 12

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