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Faculté des Sciences
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Evaluation of the bioactive potential of polar cyanobacterial strains

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Picone, Dorian ULiège
Promotor(s) : Wilmotte, Annick ULiège ; Vaz, Marcelo ULiège
Date of defense : 29-Jun-2023 • Permalink : http://hdl.handle.net/2268.2/17088
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Title : Evaluation of the bioactive potential of polar cyanobacterial strains
Translated title : [fr] Évaluation du potentiel bioactif de souches de cyanobactéries polaires
Author : Picone, Dorian ULiège
Date of defense  : 29-Jun-2023
Advisor(s) : Wilmotte, Annick ULiège
Vaz, Marcelo ULiège
Committee's member(s) : Rigali, Sébastien ULiège
Frederich, Michel ULiège
Becker, Pierre 
Language : English
Number of pages : 46
Discipline(s) : Life sciences > Microbiology
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en biochimie et biologie moléculaire et cellulaire, à finalité approfondie
Faculty: Master thesis of the Faculté des Sciences

Abstract

[en] Antibiotic and antifungal resistance has become a major public health problem worldwide, with significant clinical and economic consequences. It is therefore imperative to take rapid and sustained action over the coming years to tackle this situation. Cyanobacteria, oxygenic photosynthetic prokaryotes, offer promising potential in many fields such as pharmaceuticals, biotechnology, cosmetics, food, and many others. Previous research has identified numerous strains capable of producing antimicrobial compounds. These could represent a new avenue of research in the fight against the emergence of drug resistance.
As part of this thesis, an evaluation of 41 polar cyanobacterial strains from the BCCM/ULC Collection was undertaken to study their activity against pathogenic bacteria and fungi. In addition, a molecular analysis was carried out to determine their genetic potential to produce cyanotoxins and protease-inhibiting peptides, enabling us to better characterize their safety or potential usefulness in other clinical fields.
The results of this study highlighted the strains Phormidesmis priestleyi ULC007 and Microcoleus attenuatus ULC371, whose methanolic extracts demonstrated significant antifungal activity against Candida albicans and Aspergillus fumigatus.
The study also revealed the presence of the mcyE gene, involved in microcystin biosynthesis, in Nostoc spp. strains ULC008, ULC399 and ULC447. It also revealed the presence of a specific intergenic region between the mcnC and mcnE genes, involved in cyanopeptolin biosynthesis, in the strain Wilmottia murrayi ULC376.
These results open up new prospects for further research aimed at isolating and identifying the compound(s) responsible for the observed antifungal activity. They also deepen our understanding of the biosynthetic potential of microcystin and cyanopeptolin in these strains. These advances could eventually lead to the development of promising new therapeutic applications.


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Author

  • Picone, Dorian ULiège Université de Liège > Master bioch. & biol. mol. & cel., à fin.

Promotor(s)

Committee's member(s)

  • Rigali, Sébastien ULiège Université de Liège - ULiège > Département des sciences de la vie > Centre d'Ingénierie des Protéines (CIP)
    ORBi View his publications on ORBi
  • Frederich, Michel ULiège Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
    ORBi View his publications on ORBi
  • Becker, Pierre
  • Total number of views 44
  • Total number of downloads 2










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