Thesis, COLLÉGIALITÉ

Abstract

The use of viral vectors in research and industry has been growing steadily for several years. Viral vectors are derived from viruses that have been modified to eliminate their virulence while retaining their ability to penetrate cells. Retroviral vectors, including lentiviruses, integrate their genomic material into the genome of the target cell, while adeno-associated vectors replicate in the cytoplasm without permanent integration. These vectors have a wide range of applications, from the treatment of various diseases, such as cancer or diseases of genetic origin, to the study of signaling pathways and protein interactions in fundamental research. Although highly effective, these vectors have a lengthy development process, resulting in substantial production costs that are sometimes difficult to make profitable. Optimization of the various production stages is necessary to reduce R&D costs and accelerate viral vector production, so that their use is viable on a large scale. The aim of the work carried out in this thesis was to optimize various stages of the AAV and lentivirus production process.