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Faculté de Médecine
Faculté de Médecine
MASTER THESIS
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Thesis, COLLÉGIALITÉ

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Rossdeutsch, Ludivine ULiège
Promotor(s) : Kempf, Anissa
Date of defense : 3-Jul-2023 • Permalink : http://hdl.handle.net/2268.2/17926
Details
Title : Thesis, COLLÉGIALITÉ
Translated title : [fr] Étude du rôle de dSUR dans la régulation du sommeil
Author : Rossdeutsch, Ludivine ULiège
Date of defense  : 3-Jul-2023
Advisor(s) : Kempf, Anissa 
Committee's member(s) : Vandewalle, Gilles ULiège
Dejardin, Emmanuel ULiège
Schmidt, Christina ULiège
Nguyen, Laurent ULiège
Language : English
Number of pages : 65
Keywords : [en] Sleep regulation
[en] Sleep duration
[en] dSUR
[en] K (ATP) channel
[en] PAM neurons
[en] Mushroom Body
[oth] Drosophila melanogaster
Discipline(s) : Life sciences > Anatomy (cytology, histology, embryology...) & physiology
Funders : Université de Liège, , Biozentrum Basel, University of Basel
Research unit : Biozentrum, Kempf group
Target public : Researchers
Professionals of domain
Student
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en sciences biomédicales, à finalité approfondie
Faculty: Master thesis of the Faculté de Médecine

Abstract

[en] Sleep is highly conserved across species and lack of it, as well as excess, can lead to multiple symptoms and diseases. Although it is essential to our survival, little is known about its mechanisms of regulation. In 2013, a genome-wide association study conducted by Allebrandt et al. showed a significant correlation between SUR2 polymorphism and sleep duration in humans. SUR2 is a regulatory subunit to an ATP-sensitive potassium channel and acts as a sensor of cellular energy metabolism. Pan-neuronal knock-down of its Drosophila homolog, dSUR, shows a significant sleep decrease. Our goal is to decipher dSUR-mediated sleep regulation by investigating the neuronal populations through which it acts and exploring its effect on sleep in Drosophila melanogaster. To this aim, we performed dSUR silencing using dSUR RNAi in different neuronal populations. We identified dopaminergic inputs to the mushroom body, the PAM neurons, to be the principal actors in dSUR-mediated sleep regulation. The mushroom body is a neuropil shown to play a role in associative learning and sleep. We performed a wide screening of the PAM neurons and identified subsets of them being wake-promoting and others being sleep-promoting. Moreover, we used MiMIC lines to localize dSUR expression in the Drosophila brain and found dSUR-positive neurons close to the mushroom body. Together, these results shed light on a potentially crucial actor in sleep regulation, SUR2, and its role in sleep homeostasis through dopaminergic circuitry. Further investigations will be needed to better understand its mechanisms of action and its eventual role as a linker between sleep and metabolism.


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Author

  • Rossdeutsch, Ludivine ULiège Université de Liège > Master sc. bioméd., à fin.

Promotor(s)

Committee's member(s)

  • Vandewalle, Gilles ULiège Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
    ORBi View his publications on ORBi
  • Dejardin, Emmanuel ULiège Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
    ORBi View his publications on ORBi
  • Schmidt, Christina ULiège Université de Liège - ULiège > Département de Psychologie > Neuropsychologie
    ORBi View his publications on ORBi
  • Nguyen, Laurent ULiège Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
    ORBi View his publications on ORBi
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