Faculté des Sciences
Faculté des Sciences

Streptomyces scabiei, only a plant pathogen? Understanding the antagonism between Streptomyces scabiei and Phytophthora infestans

Kerdel, Yasmine ULiège
Promotor(s) : Rigali, Sébastien ULiège
Date of defense : 4-Sep-2023 • Permalink :
Title : Streptomyces scabiei, only a plant pathogen? Understanding the antagonism between Streptomyces scabiei and Phytophthora infestans
Translated title : [fr] Streptomyces scabiei, seulement un pathogène végétal ? Compréhension de l'antagonisme entre Streptomyces scabiei et Phytophthora infestans.
Author : Kerdel, Yasmine ULiège
Date of defense  : 4-Sep-2023
Advisor(s) : Rigali, Sébastien ULiège
Committee's member(s) : Ongena, Marc ULiège
Hanikenne, Marc ULiège
Tocquin, Pierre ULiège
Language : English
Number of pages : 50
Keywords : [en] Antagonism, siderophore, Streptomyces scabiei, Phytophthora infestans
Discipline(s) : Life sciences > Microbiology
Target public : Other
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en biochimie et biologie moléculaire et cellulaire, à finalité approfondie
Faculty: Master thesis of the Faculté des Sciences


[en] So far, the study of the interaction between potato phytopathogens and their host has primarily focused on their virulence determinants and their infection mechanisms. In this study, our aim is to understand the interactions between two pathogens (Streptomyces scabiei and Phytophthora infestans) that infect the same plant host (Solanum tuberosum). A prior study illustrated the antagonistic effects of S. scabiei 87-22 against P. infestans. The inhibition increased proportionally to the amount of peptone added in the medium. A comparative metabolomic study revealed that desferrioxamines (siderophores) are suggested to be the best candidates responsible for this antagonism.
The first objective of this master’s thesis is to identify the molecules by which S. scabiei inhibits the growth of P. infestans and determine whether this characteristic is also shared by other members of the Streptomyces genus.
The second objective is to unveil the environmental elicitors that trigger the production of these antagonistic compounds.
Main results:
Through our experiments to answer the first question, we demonstrated that by targeting iron homeostasis (by depleting or replenishing the media with iron), we could modulate the growth inhibition of P. infestans, meaning that siderophores play a key role in this antagonism by competing for this essential nutrient, thus depriving P. infestans and inhibiting its growth. We also observed that this strategy was widespread amongst Streptomyces species.
To achieve the second objective, we substituted peptone (a nitrogen source used for synthetic media preparations in labs) with nitrogen sources commonly used for soil amendment in agriculture. We demonstrated that nitrogen sources triggered the production of siderophores and induced growth inhibition of P. infestans. However, the mechanism by which nitrogen sources control siderophore production remains unknown.



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  • Kerdel, Yasmine ULiège Université de Liège > Master bioch. & biol. mol. & cel., à fin.


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