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Characterization of the interaction between Varicella-Zoster virus (VZV) ORF9 protein and the major transactivator IE62

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Sanchez Gil, Judit ULiège
Promotor(s) : Sadzot, Catherine ULiège
Date of defense : 7-Sep-2017 • Permalink : http://hdl.handle.net/2268.2/3162
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Title : Characterization of the interaction between Varicella-Zoster virus (VZV) ORF9 protein and the major transactivator IE62
Translated title : [fr] Caractérisation de l'interaction entre la protéine ORF9 du virus Varicelle et du zona (VZV) et le transactivateur majeur IE62
Author : Sanchez Gil, Judit ULiège
Date of defense  : 7-Sep-2017
Advisor(s) : Sadzot, Catherine ULiège
Committee's member(s) : Lebrun, Marielle ULiège
Machiels, Bénédicte ULiège
Thiry, Marc ULiège
Dequiedt, Franck ULiège
Language : English
Number of pages : 45
Keywords : [en] VZV, ORF9p, IE62, phosphorylation, secondary envelopment
Discipline(s) : Human health sciences > Immunology & infectious disease
Target public : Researchers
Professionals of domain
Student
Institution(s) : Université de Liège, Liège, Belgique
Degree: Master en biochimie et biologie moléculaire et cellulaire, à finalité approfondie
Faculty: Master thesis of the Faculté des Sciences

Abstract

[en] In Varicella-zoster virus (VZV) the most expressed protein during infection is ORF9p.
This protein is conserved in all the Alphaherpesvirus and is an essential tegument
protein. ORF9p interacts with IE62 the major VZV transactivator which is also part
of the tegument although the mechanisms leading to its incorporation into the
particle are poorly understood. Considering that both proteins are present in high
quantity in the viral tegument, it is suggested that one of the ORF9p functions is the
recruitment of IE62 in order to be incorporated into the tegument (Cilloniz et al.,
2007). Previous studies done in the laboratory prove that ORF9p plays an important
role during secondary envelopment and viral particle formation. It is suggested that
this main function is regulated by its phosphorylation and the interaction with other
viral proteins (Riva et al., 2013).
The first goal of this work is to determine if the phosphorylation of ORF9p is
important for the interaction with IE62. The second aim is to determine the
responsible site of ORF9p for the interaction with IE62 and glycoprotein gE.
The role of ORF9p phosphorylation in the interaction with IE62 has been analysed
using different ORF9p mutants lacking a phosphorylation by the ORF47 viral kinase.
On one hand, we have demonstrated that the interaction between ORF9p and IE62
does not depend on the ORF9p phosphorylation level. On the other hand, we
observe a co-localisation of both proteins accumulate in subcellular structures that
accumulates in the vicinity of the nucleus and whose nature has still to be
determined. More broadly, using mutants unable to express ORF47p and/or
ORF66p, the two ser/thr kinases encoded by the virus or-phosphatase
treatments, we showed that nor the phosphorylation of ORF9p or IE62 was
important for their interaction.
In a second part, we evaluate the importance of an WW domain present ORF9p
sequence and shown to be important for HSV-1 VP22 (ORF9p homologue)
interaction with VP16 the major transactivator. ORF9p punctual mutants were
created by mutating the two tryptophan and the phenylalanine described to be
important in HSV-1. Interestingly, F181A and W201F mutations present a loss of
infection over time. Therefore, these two amino acids are perhaps important for
the interaction with IE62 and gE.
These observations confirm that nor the phosphorylation of ORF9p and IE62 or the
amino acids F181 and W201 of ORF9p, located in a putative WW domain are not
important for the interaction between these 2 tegument proteins.


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Author

  • Sanchez Gil, Judit ULiège Université de Liège > Master bioch. & biol. mol. & cel., à fin.

Promotor(s)

Committee's member(s)

  • Lebrun, Marielle ULiège Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie et immunologie
    ORBi View his publications on ORBi
  • Machiels, Bénédicte ULiège Université de Liège - ULg > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
    ORBi View his publications on ORBi
  • Thiry, Marc ULiège Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie cellulaire
    ORBi View his publications on ORBi
  • Dequiedt, Franck ULiège Université de Liège - ULg > Département des sciences de la vie > Génétique et biologie moléculaires animales
    ORBi View his publications on ORBi
  • Total number of views 42
  • Total number of downloads 14










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