Développement analytique d'un « screening » volatolomique pour applications médicales

Abstract

This master thesis is divided in two parts. The first part of this work is the evaluation of Thermal Desorption tubes (TDTs) in order to improve breath analyses. To obtain comprehensive overview of the sample conditions, the most effective adsorbent has to be selected. In this study, six different sorbent tubes were evaluated using a well-defined standard mixture and exhaled breath analysis after coffee ingestion. The second part focus on the study of Volatile Organic Compounds (VOCs) produced from oxidized epithelial cells. To better understand the metabolic pathway modifications during lung inflammation, an in-vitro model was developed using lung epithelial cell culture. In this work, the culture conditions (normal and oxidized) were established to make them compatible with VOC screening. Following that step, the analytical conditions were established. Finally, a processing workflow was set up to compare the different culture conditions.